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KMID : 0606920120200040380
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Biomolecules & Therapeutics
2012 Volume.20 No. 4 p.380 ~ p.385
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Suppression of Transglutaminase-2 is Involved in Anti-Inflammatory Actions of Glucosamine in 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation
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Park Mi-Kyung
Cho Sun-A
Lee Hye-Ja
Lee Eun-Ji
Kang June-Hee
Kim You-Lee
Kim Hyun-Ji
Oh Seung-Hyun
Choi Chang-Sun
Lee Hoo-Keun
Lee Ho
Kim Soo-Youl
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Abstract
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Glucosamine (GS) is well known for the treatment of inflam-mation. However, the mechanism and efficacy of GS for skin inflammation are unclear. The aim of this study was to evaluate the effects and mechanism of GS in the mouse 12-O-tetradecanoyl 13-acetate (TPA)-induced ear edema model. TPA-induced ear edema was evoked in ICR or transglutaminase 2 (Tgase-2) (-/-) mice. GS was administered orally (10-100 mg/kg) or topically (0.5-2.0 w/v %) prior to TPA treatment. Orally administered GS at 10 mg/kg showed a 76 or 57% reduction in ear weight or myeloperoxidase, respectively, and a decreased expression of cyclooxy-genase-2 (COX-2), NF-κB and Tgase-2 in TPA-induced ear edema by western blot and immunohistochemistry. Role of Tgase-2 in TPA ear edema is examined using Tgase-2 (-/-) mice and TPA did not induce COX-2 expression in ear of Tgase-2 (-/-) mice. These observations suggested that Tgase-2 is involved in TPA-induced COX-2 expression in the inflamed ear of mice and anti-inflammatory effects of glucosamine is mediated through suppression of Tgase-2 in TPA ear edema.
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KEYWORD
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Glucosamine, TPA-induced ear edema, Transglutaminase-2, Cyclooxygenase-2, NF-κB, Tgase-2 (-/-) mice
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